Hoechst and propidium iodide
NettetDouble-staining with Hoechst and propidium iodide (PI). The images in the left column show the total number of cells stained with Hoechst, the middle column shows the cells … Nettet1. sep. 1994 · A flow cytometric method to detect apoptotic cells is described. This method is based on the detection of differences in chromatin condensation with Hoechst 33342 …
Hoechst and propidium iodide
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Nettetwhen bound to DNA, the bis-benzimidazole, Hoechst 33342, and propidium iodide. Three populations were identified and characterized. On excitation with UV radiation, dead cells fluoresced red due to the uptake of propidium iodide whereas apoptotic cells fluoresced bright blue; normal cells showed low blue, low red fluorescence. Nettet1. sep. 2016 · The rare population of CML stem cells can be identified by staining with cell surface markers, and then DNA-binding dyes Hoechst 33342 and propidium iodide …
NettetThe effects of MTDH/AEG-1 siRNA on apoptosis and morphology in three cell lines (T24, RT4, and HT1376) were measured using Hoechst 33342 (Sigma-Aldrich Co., St Louis, … NettetHoechst 33342 (from Sigma) comes as a lyophilized powder and is re-suspended at 1 mg/mL in water, filter sterilized, and frozen in small aliquots.. HBSS+ (Hanks Balanced …
Nettet17. jun. 2024 · HOECHST staining is toxic for intestinal organoids after prolonged exposure and therefore is not recommended in this protocol when measuring permeability for more than 12 h. When additionally staining with HOECHST, add HOECHST (5 mg/mL stock) to the pre-warmed intestinal organoid growth media with a final concentration of 5 μg/mL … NettetDAPI has higher photostability than Hoechst 33342. Other DNA-binding dyes can also be used including propidium iodide and ethidium bromide. Staining with propidium …
NettetWhen choosing which dye to use, consider whether the dye may cover the markers of transfection. For example, Hoechst dye, ethidium bromide (EB), and propidium iodide (PI) are compatible for GFP. Hoechst dye, however, can be used only with a light X-Gal staining, since a dark X-Gal staining will cover the fluorescence of Hoechst dye.
NettetHoechst and DAPI are popular blue fluorescent, nuclear-specific dyes that can be used to stain live or fixed cells. Both DAPI and Hoechst are minor-groove binding dyes with a preference for A/T-rich regions of DNA over G/C-rich DNA. The dyes have minimal fluorescence in solution, but become brightly fluorescent upon binding to DNA. downtown st louis hotel dealsNettetPropidium iodide is a membrane-impermeable fluorescent DNA stain. It is used to stain apoptotic cells for flow cytometry, in situ hybridization, and immunohistochemistry … cleaning bathroom extractor fanNettetPropidium iodide (PI) and Hoechst 33342 double staining in cultured C6 cells 72 hours after 400 μM ganciclovir (GCV) administration (×400). Cells emitting blue fluorescence … cleaning bathroom fans petaluma caNettetPropidium iodide (PI) and Hoechst 33342. 1. Prepare a 4X Master Mix staining solution: a. Pipette 5 mL of cell culture media or 1x PBS into a 15 mL centrifuge tube. b. Add 40 µl of the Propidium iodide (Component A) to the 15 mL tube. c. Add 8 µl of the Hoechst 33342 (Component B) to the 15 mL tube. d. Vortex tube for 10 seconds. 2. downtown st louis arch hotelsNettet11. apr. 2024 · Effect of tuna blood (TWBF and TRBF) on cell morphology of lung cancer cells (a) Representative immunofluorescence images of Hoechst 33342/propidium iodide staining on A549 cells after exposure to TWBF. (b) Quantitative data represents the percentage of apoptotic nuclei on A549 cells after exposure to TWBF. downtown st louis hotels near archNettetVybrant Apoptosis Assay kits offer streptavidin biotin-annexin V, propidium iodide (PI), YO-PRO-1, and Hoechst 33342 dyes to differentiate live from apoptotic or necrotic … cleaning bathroom ceiling mold and mildewNettetThe cells were stained with the triple-stain Hoechst 33342 + calcein-AM + propidium iodide. The DNA stain Hoechst 33342 stains nuclei in both living and dead cells and emits blue fluorescence. Calcein-AM is cleaved by intracellular esterases to calcein, emitting green fluorescence in live cells with intact plasma membranes. downtown st louis thanksgiving day parade